Article Sidebar

Download
PDF (Bahasa Indonesia)
Statistic
Read Counter : 2 Download : 1

Downloads

Download data is not yet available.

Main Article Content

Abstract

Oral cancer ranks 12th among the most common types of cancer worldwide, with a prevalence of approximately 25%-40% occurring on the lateral borders and the underside of the tongue. Conventional therapies often cause side effects that damage normal cells, prompting the use of safer natural alternatives, such as Cymbopogon citratus (lemongrass). The active compounds in lemongrass leaves can enhance the production of reactive oxygen species (ROS), which trigger apoptosis through the depolarization of mitochondrial membrane potential (MMP). To improve the stability of these active compounds, they are encapsulated with chitosan, which is modified into nanoparticles to enhance diffusion through cell membranes, thereby increasing their effectiveness as anticancer agents. This study involved nine groups, including a negative control, a positive control (doxorubicin), nano chitosan, lemongrass extract, and five treatment groups with various encapsulation concentrations (100%, 75%, 50%, 25%, and 12.5%). The results showed that nano chitosan encapsulation of lemongrass extract significantly increased MMP depolarization compared to the control. The depolarization effect increased with higher encapsulation concentrations, with the lowest red fluorescence intensity observed at the 100% concentration, indicating the highest level of depolarization in HSC-3 cells.

Article Details

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Author Biographies

Komariah Komariah, Universitas Trisakti

Program Studi Magister Kedokteran Gigi, Fakultas Kedokteran Gigi, Universitas Trisakti

Jenyfer Ignatia Moksidy, Universitas Trisakti

Program Studi Profesi Dokter Gigi, Fakultas Kedokteran Gigi, Universitas Trisakti, DKI jakarta

Didi Nugroho, Universitas Trisakti

Program Studi Magister Kedokteran Gigi, Fakultas Kedokteran Gigi, Universitas Trisakti

Dewi Ranggaini, Universitas Trisakti

Program Studi Pendidikan Dokter Gigi, Fakultas Kedokteran Gigi, Universitas Trisakti

Rezky Anggraeni , Universitas Trisakti

Program Studi Pendidikan Dokter Gigi, Fakultas Kedokteran Gigi, Universitas Trisakti

Johni Halim , Universitas Trisakti

Program Studi Pendidikan Dokter Gigi, Fakultas Kedokteran Gigi, Universitas Trisakti
How to Cite
Komariah, K., Moksidy, J. I., Nugroho, D., Ranggaini, D., Anggraeni , R., & Halim , J. (2025). Efektivitas Enkapsulasi Nano Kitosan Serai Dapur dalam Meningkatkan Depolarisasi Mitokondria pada Sel Kanker HSC-3. Borneo Journal of Pharmascientech, 9(2), 285-295. https://doi.org/10.59053/bjp.v9i2.602
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX

References

  1. Agustin, T. 2020. Potensi metabolit aktif dalam sayuran cruciferous untuk menghambat pertumbuhan sel kanker. Jurnal Penelitian Perawat Profesiona, 2(4), 459-472.
  2. Amtha, R., Komariah, K., Priandini, D., Roeslan, M. O., Kelsi, F., Landy, R., Agustini, D., Ardiana, I. S., Krista, J. I., & Budi, K. S. A. 2022. Pelatihan deteksi dini kanker mulut dengan SAMURI pada komunitas penyintas kanker Love and Healthy Tangerang. ABDI MOESTOPO: Jurnal Pengabdian Pada Masyarakat, 5(1), 10–21.
  3. Andikoputri, S. F., Komariah, K., Roeslan, M. O., Ranggaini, D., & Bustami, D. A. 2021. Nano chitosan encapsulation of Cymbopogon citratus leaf extract promotes ROS induction leading to apoptosis in human squamous cells (HSC-3). Current Issues in Pharmacy and Medical Sciences, 34(3), 134–137.
  4. Dewi, R. K. 2020. Hubungan kepatuhan menjalani kemoterapi dengan kualitas hidup pasien kanker payudara di RSUD Dr. Moewardi Surakarta. Jurnal Ilmiah Kesehatan Masyarakat, 12(4), 158-163
  5. Elisya, Y., Achmadi, N. K., & Kurniawan, D. 2022. Overview of cytostatic drug prescribing for inpatient cancer patients at RSUD Tarakan July-December 2021 period. SANITAS: Jurnal Teknologi Dan Seni Kesehatan, 13(2), 270–282.
  6. Ericka, N., Komariah, K., Wahyudi, R., & Trisfilha, P. 2022. Nano encapsulation of lemongrass leaves extract (Cymbopogon citratus DC) on fibroblast viability with oxidative stress. In Quality Improvement in Dental and Medical Knowledge, Research, Skills and Ethics Facing Global Challenges. Proceedings of the International Conference on Technology of Dental and Medical Sciences (ICTDMS), Jakarta, Indonesia, 8–10 December 2022 (pp. 450-460). CRC Press/Balkema.
  7. Fadhlurrohman, I., Maulaeni, R., & Tirta, A. C. 2023. Fortifikasi serai (Cymbopogon citratus) pada produk susu fermentasi sebagai potensi pangan fungsional: Kajian literatur. Prosiding Seminar Nasional Pembangunan dan Pendidikan Vokasi Pertanian, 4(1), 418-421
  8. Felicia, F., Komariah, K., & Kusuma, I. 2022. Antioxidant potential of lemongrass (Cymbopogon citratus) leaf ethanol extract in HSC-3 cancer cell line. Tropical Journal of Natural Product Research, 6(4), 520–528.
  9. Firdaus, N. Z., & Susilowati, S. 2023. Evaluasi penggunaan kemoterapi pada pasien kanker payudara di Rumah Sakit Islam Sultan Agung Semarang tahun 2022. Jurnal Ilmu Farmasi dan Farmasi Klinik, 20(2), 155-166
  10. Fitria, N., Bustami, D. A., Komariah, K., & Kusuma, I. 2022. The antioxidant activity of lemongrass leaves extract against fibroblasts oxidative stress. Brazilian Dental Science, 25(4).
  11. Fitriansyah, S. N., Fadhilah, S., Ruslan, K., Hartati, R., & Fidrianny, I. 2023. Antioxidant activity and sun protection factor of different parts of Sawo Walanda (Pouteria campechiana (Kunth.) B.) extract. Indonesian Journal of Pharmaceutical Science and Technology, 5(2), 207-214.
  12. Fitriatuzzakiyyah, N., Sinuraya, R. K., & Puspitasari, I. M. 2017. Cancer therapy with radiation: The basic concept of radiotherapy and its development in Indonesia. Indonesian Journal of Clinical Pharmacy, 6(4), 311–320.
  13. Huzaifah, Z., Rahayu, S. F., Sari, R. T., & Linda, L. 2023. Hubungan kejadian anemia pada pasien kemoterapi dengan karakteristik responden berdasarkan jenis kelamin. Malahayati Nursing Journal, 5(2), 533–540.
  14. Irfan Fadhlurrohman, Ridho Maulaeni, & Asmaradika Cahya Tirta. 2023. Fortifikasi serai (Cymbopogon citratus) pada produk susu fermentasi sebagai potensi pangan fungsional: Kajian literatur. Prosiding Seminar Nasional Pembangunan Dan Pendidikan Vokasi Pertanian, 4(1), 418–428.
  15. Lathifah, S. H., Yohana, W., & Rodian, M. 2021. Neutrophil-to-Lymphocyte Ratio (NLR) as an inflammation degree marker in tongue cancer patient. Jurnal Kesehatan Gigi, 8(2), 96-108.
  16. Komariah, K., Trisfilha, P., Wahyudi, R., Erica, N., Nugroho, D., Ariesanti, Y., & Swain, S. K. 2023. Chitosan Xylotrupes gideon encapsulated lemongrass leaf ethanol extract reduces H2O2-induced oxidative stress in human dermal fibroblast. Indonesian Journal of Biotechnology, 28(4), 191–199.
  17. Komariah, K., & Astuti, L. 2012. Preparasi dan karakterisasi kitin yang terkandung dalam eksoskeleton kumbang tanduk (Xylotrupes gideon L) dan kutu beras (Sitophilus oryzae L). In Biologi, Sains, Lingkungan, dan Pembelajarannya dalam Upaya Peningkatan Daya Saing Bangsa (pp. 648-654). Prosiding Seminar Nasional IX Pendidikan Biologi, FKIP UNS.
  18. Kumala, R. 2020. Hubungan kepatuhan menjalani kemoterapi dengan kualitas hidup pasien kanker payudara di RSUD Dr. Moewardi Surakarta. Jurnal Ilmiah Kesehatan Masyarakat, 12(4), 158-163.
  19. Mursal, I. L. P., Warsito, A. M. P., Ariyanti, D. K., Susanti, E. I., & Irma, R. 2023. Penggunaan nanopartikel kitosan sebagai penghantar obat baru. Journal of Pharmaceutical and Sciences, 6(2), 804-809.
  20. Prasetyo, D. Y., & Suprayitno, E. 2021. Faktor-faktor kualitas hidup pasien kanker. Care: Jurnal Ilmiah Ilmu Kesehatan, 9(2), 322-333.
  21. Purwandari, V., Isnaeni, I., Rahmi, R., Akbari, A. Z., & Akbari, M. Z. 2022. Formulasi nanoenkapsulasi ekstrak daun kelor (Moringa oleifera)/kitosan-natrium tripolifosfat (NaTPP). Journal of Science and Applicative Technology, 6(2), 77.
  22. Sari, L. M. (2018). Apoptosis: Mekanisme molekuler kematian sel. Cakradonya Dental Journal, 10(2), 65-70
  23. Suharto, D. N. 2018. Analisis kasus kanker lidah dalam konteks asuhan keperawatan dengan pendekatan Comfort Theory Model. Poltekita: Jurnal Ilmu Kesehatan, 12(2), 88-94.
  24. Susilowati, E. 2023. Hubungan Fatigue Dengan Kualitas Hidup Pasien Ca Mammae Yang Menjalani Kemoterapi di RSUP Dr. Soeradji Tirtonegoro Klaten. Jurnal Kesehatan RSUP Dr. Soeradji Tirtonegoro Klaten, 1(1), 1-20.
  25. Timoty, H., Komariah, K., & Nugroho, D. 2023. Pengaruh partikel silver ekstrak daun serai dapur (Cymbopogon citratus DC) terhadap galur sel rongga mulut HSC-3: Penelitian eksperimen laboratorium. Jurnal Kedokteran Gigi Universitas Padjadjaran, 35(1), 78-85.
  26. Veronica, G., Komariah, K., & Maria, L. G. C. 2021. Microencapsulation of lemongrass leaves effect on reactive oxygen species (ROS) fibroblasts. InHeNce 2021 - 2021 IEEE International Conference on Health, Instrumentation and Measurement, and Natural Sciences.
  27. Zalukhu, M. L., Phyma, A. R., & Pinzon, R. T. (2016). Proses menua, stres oksidatif, dan peran antioksidan. CDK-245, 43(10), 733-734.