Article Sidebar

Download
PDF (Bahasa Indonesia)
Statistic
Read Counter : 510 Download : 323

Downloads

Download data is not yet available.

Main Article Content

Abstract

Brazilian Spinach (Althernanthera sisso) leaves are known to have a high natural antioxidant activity content of 3.7 ppm. Antioxidants have an important role in the human body to prevent damage caused by free radicals. One of the compounds that have antioxidant activity is flavonoids. Based on this description, it is necessary to know the content of total flavonoids in A. sisso This study aims to determine qualitatively using Thin Layer Chromatography (TLC), while quantitatively using UV-Vis spectrophotometry. Maceration method with 70% ethanol solvent was used to extract Brazilian spinach leaves. Thin Layer Chromatography (TLC) followed by spray reagents was used to test the flavonoid compounds. Quercetin reagent was used to determine total flavonoid levels, while AlCl3 was used to determine total flavonoid levels. The results of this qualitative test showed the presence of flavonoids, and in the quantitative test using UV-Vis spectrophotometry the total levels of flavonoids were 70.890 ± 1.723% w/w quercetin equivalent. The 70% ethanol extract of A. sisso leaves contains flavonoids so that it has the potential to be developed as a medicinal plant

Keywords

Althernanthera sisso Total flavonoids; 70% ethanol extract

Article Details

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite
Marwati, N. H., Revita Saputri, & Eka Fitri Susiani. (2024). Penetapan Kadar Flavonoid Total Ekstrak Etanol 70% Daun Bayam Brazil (Althernanthera sisso) Dengan Metode Spektrofotometri UV-Vis. Borneo Journal of Pharmascientech, 8(1), 98-105. https://doi.org/10.51817/bjp.v8i1.496
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX

References

  1. Afianti, S. 2022. Farmakologi Jambu Biji dalam Prespektif Terapi Periodontitis. Literasi Nusantara Abadi, Malang.
  2. Anuar, A. K. K. 2022. A comparative study on phytochemical constituents and antimicrobial activity of Alternanthera sissoo extracts. Enhanced Knowledge in Sciences and Technology, 2(2), 043-051.
  3. Azizah, Z., Elvis, F., Zulharmita, Z., Misfadhila, S., Chandra, B., & Yetti, R. D. 2020. Penetapan Kadar Flavonoid Rutin pada Daun Ubi Kayu (Manihot Esculenta Crantz) Secara Spektrofotometri Sinar Tampak. Jurnal Farmasi Higea, 12(1), 90-98.
  4. Dewi, N. W. R. K., I Wayan, G & Ni Made, P. 2017. Isolasi dan identifikasi Senyawa antioksidan golongan flavonoid dari ekstrak etil asetat daun Pranajiwa (Euchresta horsfieldii Lesch Benn.) Cakra kimia. 5: 1-9.
  5. Kemenkes RI. 2017. Farmakope Herbal Indonesia Edisi 2. Kementerian Kesehatan Republik Indonesia, Jakarta.
  6. Lindawati, N. Y., & Ma'ruf, S. H. 2020. Penetapan kadar flavonoid total ekstrak etanol kacang merah (Phaseolus vulgaris L.) secara spektrofotometri visibel. Jurnal Ilmiah Manuntung, 6(1), 83-91.
  7. Maryani, E. 2015. Uji Aktivitas Antioksidan Ekstrak Daun Bayam Hijau (Amaranthus cruentus L.) Dan Daun Bayam Merah (Amaranthus tricolor L.) Dengan Metode DPPH. Bandung : Kementerian Kesehatan RI Politeknik Kesehatan Bandung Jurusan Farmasi.
  8. Parwata, I. M. O. A., Kusuma, I. N. A., & Dewi, I. G. A. K. S. P. 2022. Kadar flavonoid total Dan Uji Aktivitas Antioksidan Fraksi Etil Asetat Daun Gaharu (Gyrinops versteegii). Jurnal Kimia (Journal of Chemistry), 16(1).
  9. Rahayu, N. K. S. I. 2022. Skrining Fitokimia dan Aktivitas Antioksidan Ekstrak Etanol Bayam Brazil (Althernanthera sisso). Skripsi. Poltekkea Kemenkes Denpasar Jurusan Teknologi Laboratorium Medis.
  10. Sommai, S., Cherdthong, A., Suntara, C., So, S., Wanapat, M., & Polyorach, S. 2021. In vitro fermentation characteristics and methane mitigation responded to flavonoid extract levels from Alternanthera sissoo and dietary ratios. Fermentation, 7(3), 109.
  11. Wuni. P. M, Madyaningrana. K, Prakasita. C.V. 2022. Efek Ekstrak Daun Bayam Brazil (Althernanthera sisso hort) Terhadap Jumlah Limfosit dan Indeks Organ Timus dan Limpa Mencit Jantan. Metamorfosa ; Journal of Biological Sciences. Yogyakarta. 9 (2):397-406.
  12. Yuliani, C. R., H. Ramadhan., P. I. Sayakti., C. Torizellia. 2022. Kadar Total Fenolik dan Flavonoid Fraksi N-Heksan Daun Binjai (Mangifera caesia Jack. Ex Wall). Jurnal Ilmiah Farmasi. 11-19.