ISOLASI ACTINOMYCETES PENGHASIL ANTIBIOTIK TERHADAP Escherichia coli DAN Staphylococcus aureus DARI TANAH SAWAH

  • Hafiz Ramadhan STIKES Borneo Lestari Banjarbaru hafizramadhan14@gmail.com

Abstrak

ISOLASI ACTINOMYCETES PENGHASIL ANTIBIOTIK TERHADAP Escherichia coli DAN Staphylococcus aureus DARI TANAH SAWAH Oleh Hafiz Ramadhan STIKES Borneo Lestari Banjarbaru hafizramadhan14@gmail.com ABSTRACT The purpose of this study is to obtain Actinomycetes isolates that have the potential effect of producing antibiotics from the paddy soil planted with rice, corn and sugar cane and also to determine the number of Actinomycetes isolates obtained from the paddy soil. The soil samples were taken from three different paddy soil planted with rice, corn and sugar cane consecutively in the area of Bantul, Yogyakarta. From this study, we found 30 isolates, that are 19 isolates derived from paddy soil of rice, 4 isolates from paddy soil of corn and 7 isolates from paddy soil of sugar cane, which the 30 isolates can be classified into 4 groups based on color grouping. Based on the results of the bacterial inhibition test using the agar block method, it is found that among the 30 isolates there are 4 isolates that can potentially produce antibiotics. Two isolates were only able to inhibit Staphylococcus aureus, that are isolates P 301 with a diameter of 20.00 mm barrier region were categorized as strong, and isolates P 302 of 16.00 mm were categorized as medium. The remaining two isolates, P 103 and T 101 has the ability to inhibit the growth of Staphylococcus aureus and Escherichia coli as well. Isolates P 103 has a diameter of 10.00 mm zone of inhibition and T 101 of 11.00 mm were categorized as medium in inhibiting Staphylococcus aureus. While in inhibiting Escherichia coli, isolates T 101 (13.00 mm) were categorized as medium and isolates P 103 (6.00 mm) were categorized as weak. Based on these results we can conclude that the Actinomycetes isolated from soil of rice fields, corn and sugar cane can produce potential antibiotic with a spectrum of work: 2 isolates inhibited Gram-positive bacteria and the other two isolates inhibited Gram-positive and also Gram-negative bacteria.
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2019-02-28